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PUBLIC RELEASE DATE: 25-Oct-2013
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ShareContact: Robert Redmond
rredmond@cshl.edu
516-422-4101
Cold Spring Harbor Laboratory
Cell culture systems for specific neural cell types are essential for studies of their development and function.
Purifying and Culturing Neural Cells: A Laboratory Manual provides step-by-step protocols for isolating specific cell populations from rodent tissues and culturing them under conditions that closely resemble those in vivo. The contributors describe in detail how to dissect the brain, spinal cord, and other tissues; how to separate cells using mechanical and enzymatic tissue-dissociation strategies; the use of immunopanning and fluorescence-activated cell sorting (FACS) to enrich the target cell population; and the culture conditions that optimize cell viability and growth. Retinal ganglion cells, motor neurons, dorsal root ganglion cells, astrocytes, oligodendrocytes, and Schwann cells are covered, as are vascular cells such as pericytes and endothelial cells. Myelinating co-cultures of neurons and oligodendrocytes are also described.
The manual includes detailed recipes for media and reagents, tips for avoiding common pitfalls, and advice for designing new immunopanning protocols using tissues from other sources. Many of the protocols are accompanied by freely accessible online movies that demonstrate critical steps of the procedures. This is an essential laboratory companion for all neurobiologists, from the graduate student level upwards.
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AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.
PUBLIC RELEASE DATE: 25-Oct-2013
[
]
ShareContact: Robert Redmond
rredmond@cshl.edu
516-422-4101
Cold Spring Harbor Laboratory
Cell culture systems for specific neural cell types are essential for studies of their development and function.
Purifying and Culturing Neural Cells: A Laboratory Manual provides step-by-step protocols for isolating specific cell populations from rodent tissues and culturing them under conditions that closely resemble those in vivo. The contributors describe in detail how to dissect the brain, spinal cord, and other tissues; how to separate cells using mechanical and enzymatic tissue-dissociation strategies; the use of immunopanning and fluorescence-activated cell sorting (FACS) to enrich the target cell population; and the culture conditions that optimize cell viability and growth. Retinal ganglion cells, motor neurons, dorsal root ganglion cells, astrocytes, oligodendrocytes, and Schwann cells are covered, as are vascular cells such as pericytes and endothelial cells. Myelinating co-cultures of neurons and oligodendrocytes are also described.
The manual includes detailed recipes for media and reagents, tips for avoiding common pitfalls, and advice for designing new immunopanning protocols using tissues from other sources. Many of the protocols are accompanied by freely accessible online movies that demonstrate critical steps of the procedures. This is an essential laboratory companion for all neurobiologists, from the graduate student level upwards.
###
[
Share]
AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.
Source: http://www.eurekalert.org/pub_releases/2013-10/cshl-nmf102513.php
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